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產品名稱：TCEP HCl, Tris（2-Carboxyethyl） Phosphine Hydrochloride
產品貨號：PIERCE 20490
產品規格：1 GM
產品價格：566
產品說明書地址：http://www.piercenet.com/

Thermo Scientific Pierce TCEP-HCl is pure, crystalline Tris（2-carboxyethyl）phosphine hydrochloride, a thiol-free compound that is highly effective at reducing protein and peptide disulfide bonds, as well as being odorless and stable.

TCEP selectively and compley reduces even the most stable water-soluble alkyl disulfides over a wide pH range. Reductions frequently require less than 5 minutes at room temperature. TCEP is non-volatile, odorless, and unlike most other reducing agents, is resistant to air oxidation. Compared to DTT, TCEP is more stable, more effective, and able to reduce disulfide bonds at lower pHs.

Highlights:

• Odorless – reduce proteins at your desktop; contributes to a healthier lab environment
• Specific – selective and complete reduction of even the most stable water-soluble disulfides
• Simple – effective reduction at room temperature and pH 5 in less than five minutes
• Stable – resistant to air oxidation; nonvolatile and nonreactive toward other functional groups found in proteins

Advantages of TCEP-HCl over traditional alternatives for reducing disulfides:

• Odorless – Unlike DTT or BME, TCEP is odor-free, so reductions can be carried out conveniently on the bench top.
• Stable in air – The inherent stability of the TCEP moiety eliminates the need for any special precautions to avoid oxidation when handling, using or storing TCEP.
• Efficient – For most applications, 5-50 mM TCEP provides sufficient molar excess to effectively reduce peptide or protein disulfide bonds within a few minutes at room temperature.
• Compatible – With TCEP, removal of the reducing agent is not necessary prior to most applications, （e.g. histidine-tagged protein purification, maleimide conjugations）.

Product Details:

The ability of trialkylphosphine compounds to reduce protein disulfide bonds have been known for many years.^1,2 Phosphines are stable in aqueous solution, selectively reduce disulfide bonds, and are essentially nonreactive toward other functional groups commonly present in proteins.² Trialkylphosphines, however, were hindered by their instability in water and their disagreeable odor. These obstacles were overcome by discovery of tris（2-carboxyethyl）phosphine （TCEP）.^3-17