Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

These products include IgG Binding Buffers specific for each immunoglobulin-binding protein （Protein A, Protein G, Protein A/G）. The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.

The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies （or other proteins） to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer （pH 8）, which precipitates with the Gentle Ag/Ab Elution Buffer.

Highlights:

• Ready to use – carefully formulated and prefiltered 1X solutions
• Three binding buffers – optimized for Protein A, Protein G or Protein A/G
• Two elution buffer options – acidic （pH 2.8） or high salt （near-neutral）
• Versatile – elution buffers are appropriate for antibody-antigen affinity purification

Item-Specific Features:

• Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA
• Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA
• Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide
• IgG Elution Buffer: pH 2.8; amine-based
• Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide
• Gentle Ag/Ab Elution Buffer: pH 6.6; high salt （ionic strength

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