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EZ-Link（R） NHS-PEG4-Biotin,No-Weigh（tm） Format介紹

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EZ-Link（R） NHS-PEG4-Biotin,No-Weigh（tm） Format品牌介紹

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EZ-Link（R） NHS-PEG4-Biotin,No-Weigh（tm） Format

僅用于科研

Thermo Scientific EZ-Link NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.

The N-hydroxysuccinimide ester （NHS） group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The hydrophilic polyethylene glycol （PEG） spacer arm of this biotinylation reagent imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution （read more about PEGylation）. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

Highlights:

• Label almost any protein with biotin to facilitate immobilization, purification or detection
• Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
• Reagent is water soluble and membrane impermeable – suitable for cell surface labeling
• PEGylation increases solubility and helps prevent aggregation of labeled molecules
• Long spacer arm reduces steric hindrance when binding to avidin molecules
• No-Weigh Format saves time, preserves reagent activity and prevents cross-contamination between uses
• Reactive group: N-hydroxysuccinimide ester （NHS ester）

• Reactive toward: primary amines, as in side chain of lysine （K） and N-terminus of polypeptides