Thermo Scientific Pierce Traut's Reagent （2-iminothiolane） is a small thiolation compound that reacts with primary amines （e.g., lysine side chains） to add a small spacer arm （8.1 angstroms） terminated by a free sulfhydryl group.

Traut's Reagent （2-iminothiolane or 2-IT） is a cyclic thioimidate compound for thiolation （sulfhydryl addition）. Traut's Reagent reacts with primary amines （–NH2） to introduce sulfhydryl （–SH） groups while maintaining charge properties similar to the original amino group. Once added, sulfhydryl groups can be specifically targeted for reaction in a variety of useful labeling, crosslinking and immobilization procedures.

Highlights:

• Simple – one-step conversion of amines to sulfhydryls
• Specific – selective and spontaneous modification of primary amines at pH 7-10
• Flexible – incorporates a five-atom spacer arm to reduce steric hindrance
• Soluble – preserves the original positive charge to preserve protein solubility