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Pierce（R） Biotin Quantitation Kit介紹

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

Pierce（R） Biotin Quantitation Kit現貨供應

Pierce（R） Biotin Quantitation Kit品牌介紹

美國Pierce公司（www.pierce.com）是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要產品包括：

1）化學發光體和比色底物

2）分析試劑

3）熒光標記探針

4）酶聯免疫分析試劑盒等

Pierce（R） Biotin Quantitation Kit僅用于科研

Thermo Scientific Pierce Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.

HABA dye （4'-hydroxyazobenzene-2-carboxylic acid） is a reagent that enables quick estimation of the mole-to-mole ratio of biotin to protein in a solution. The Pierce Biotin Quantitation Kit contains a premix of HABA and avidin and a biotinylated horseradish peroxidase （HRP） positive control. The HABA-Avidin Premix is supplied in convenient Thermo Scientific No-Weigh Microtube packaging, which eliminates the difficulties associated with weighing small quantities of reagent.

Highlights:

• Colorimetric - requires a plate reader or spectrophotometer set to measure at 500nm
• Reliable - proven, well-characterized method for determination of biotinylation levels
• Convenient - kit provides the essential reagents and standards to perform the assay with ease
• Flexible - adaptable to spectrophotometer cuvettes or standard plate readers with 96-well microplates
• Options - calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions or use our online HABA calculator
• Robust - supplied HABA-avidin complex can be used over a wide range of pH and salt concentrations