The Thermo Scientific Pierce Secondary Antibodies featured here are affinity-purified polyclonal antibodies with well-characterized specificity for human immunoglobulin classes (IgG, IgM, IgA).

Each of these anti-human immunoglobulin polyclonal antibodies is affinity-purified using its target antigen (i.e., the target human primary antibody class, subclass and fragment) coupled to an agarose support. Affinity purification eliminates nonspecific antibodies, resulting in high specificity and low background. The purification process involves an elution procedure, that yields antibodies with high avidity. Conjugated antibodies are affinity-purified before being labeled and contain bovine serum albumin as a stabilizer.

Selected secondary antibodies have been further purified to minimize cross-reactivity to serum proteins of other species. This purification is accomplished by adsorbing the antigen-purified antibody to an affinity column containing immobilized serum proteins of selected species. This additional processing minimizes antibody cross-reactivity to the serum of proteins of the species indicated by the code "min x Sp Sr Prot," where the cross-absobed species are indicated by the accompanying abbreviation key.