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Guardian (tm) Peroxidase Conjugate Stabilizer Diluent 介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

Guardian (tm) Peroxidase Conjugate Stabilizer Diluent 現(xiàn)貨供應(yīng)

Guardian (tm) Peroxidase Conjugate Stabilizer Diluent 品牌介紹

美國(guó)Pierce公司(www.pierce.com)是蛋白質(zhì)化學(xué)、免疫學(xué)和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要產(chǎn)品包括：

1)化學(xué)發(fā)光體和比色底物

2)分析試劑

3)熒光標(biāo)記探針

4)酶聯(lián)免疫分析試劑盒等

Guardian (tm) Peroxidase Conjugate Stabilizer Diluent 僅用于科研

There's no need to worry about your antibody or streptavidin peroxidase conjugates becoming inactive over time when you use new Guardian Peroxidase Stabilizer/Diluent (S/D), which preserves peroxidase activity from 1:100 to 1:100,000 dilution (Figures 1 and 3). Guardian S/D Formulation can be used to create stock solutions (1:1,000) or ready-to-use assay reagents (1:100,000) that can be stored at 4°C for 12 months or at room temperature for six months. No more wasting money with HRP dilutions you throw away the same day.

This Guardian Formulation is ideal as an assay diluent in ELISA and solution-based assays. If you are using a membrane-based assay, Pierce recommends diluting the Guardian-stabilized HRP conjugate further in assay buffer. Figure 2 demonstrates the activity of a streptavidin-HRP conjugate stored in Guardian Stabilizer/Diluent at a concentration of 10µg/mL. This HRP conjugate is further diluted (1:300) to perform the Northern blot assay. The Streptavidin-HRP is still able to detect a biotinylated RNA probe even after 12 months of storage at 4°C at a highly dilute concentration.

Highlights:

• Preserves HRP activity – no significant loss of HRP activity over a six-month period at room temperature (1:1,000 dilution) or 12 months at 4°C
• Convenient – store ready-to-use dilutions (1:1,000-1:100,000) that maintain enzyme activity in the refrigerator – no aliquoting or freezing
• Assay compatible – simply add your favorite blocking buffer to create the ideal diluent for your HRP-based ELISA system or store the HRP conjugate as a 1:1,000 stock solution for Western blots and dilute in the final assay buffer.
• Saves money – less expensive than competitor formulations or ordering new HRP conjugates