Thermo Scientific Pierce Protein-Free Blocking Buffers are PBS and TBS formulations of a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.

Traditional blocking buffers contain proteins that can cross-react with immunodetection systems, resulting in high background and reduced signal. Typical commercial alternatives to protein-based blocking agents are ineffective. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes and other surfaces for ELISA, Western blotting, glass slide arrays and other applications.

Highlights:

• Protein-free blocker – minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
• Application-compatible – effective in all kinds of protein detection systems, including Western blots (membranes), ELISA (microplates) and arrays (coated glass slides)
• Streptavidin-friendly – absoluy free of biotin; no possible interference with avidin-biotin detection systems
• High-performance – optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)
• Convenient – supplied as ready-to-use 1X formulations in TBS and PBS with and without 0.05% Tween*-20