The Thermo Scientific Pierce IgM Purification Kit is an easy-to-use affinity matrix and buffer system to purify IgM from mouse ascites fluid; the method uses immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.

Pierce MBP Agarose is purified mannan binding protein (also called mannose-binding lectin, MBL) that has been immobilized on 4% beaded agarose. When used with an optimized buffer system based on temperature- and calcium-dependent binding, this resin enables specific affinity purification of mouse IgM from ascites fluid and other concentrated samples. The resin and essential buffers are available individually or bundled together in a complete IgM Purification Kit containing a pre-packed 5mL column of the MBP Agarose. Immobilized mannan binding protein also is available as Thermo Scientific UltraLink Resin, a durable polyacrylamide support that is compatible with high flow rates and pressures.

Highlights:

• Immobilized MBP – affinity system based on mannan binding protein (MBP) allows specific purification of mouse IgM from ascites fluid or partial isolation of human IgM from serum
• Two resin options – choose 4% beaded agarose resin or polyacrylamide-based UltraLink Resin format to suit your chromatography system
• Complete kit – IgM Purification Kit includes a pre-packed MBP column and ready-to-use buffers for convenient purification of at least 1.5mg IgM from 0.5mL ascites samples per column-use
• Mild conditions – nondenaturing, near-neutral buffer conditions ensure recovery of functional antibodies
• Reusable – stable ligand and resins can be regenerated and reused at least 10 times without significant decline in binding capacity