Single-chain polypeptide growth factor that plays a significant role in the

process of wound healing and is a potent inducer of angiogenesis. It binds

 to heparin, which potentiates its biological activity and protects it from

proteolysis. The growth factor is an extremely potent inducer of DNA synthesis

in a variety of cell types from mesoderm and neuroectoderm lineages, and

also has chemotactic and mitogenic activities. It was originally named acidic

fibroblast growth factor based upon its chemical properties and to distinguish

it from basic fibroblast growth factor.
Other homologous FGF belonging to the same family are int-2 （FGF-3 ）, FGF-5 ,

FGF-6 , K-FGF and KGF （keratinocyte growth factor =FGF-7 ）. All factors are

products of different genes, some of which are Oncogene products （FGF-3 ,

FGF-4 , FGF-5 ）.

 

Recombinant Human FGF-a （FGF-1） produced in Sf9 insect cells is a single,

glycosylated, polypeptide chain containing 140 amino acids and having a

molecular mass of 15803 Dalton.
Recombinant Human FGF-a is purified by proprietary chromatographic

 techniques.

 

The sterile protein solution （1.8mg/ml） contains 20mM Tris HCl pH=7.9,

100mM KCl, 0.2mM DTT and 20% glycerol.

 

Recombinant Human FGF-a although stable at 4⁰C for 3 weeks, should be

stored desiccated below -18⁰C. For long term storage it is recommended to

add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 

The sequence of the first five N-terminal amino acids was determined and

was found to be Met-Phe-Asn-Leu-Pro.

 

ProSpec’s Recombinant Human FGF-a is fully biologically active when compared

to standards. The ED₅₀, calculated by the dose-dependant proliferation of BAF3

 cells expressing FGF receptors （measured by ³H-thymidine uptake） is <10 ng/ml, corresponding to a specific activity of 10⁵ Units/mg.

 

Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of FGF-a as a Reference

Standard.

 

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The

product may not be used as drugs, agricultural or pesticidal products, food

additives or household chemicals.