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重組鼠纖維原生長因子-9( rmFGF-9)
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  • 更新時間:2025-05-02
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CYT-349重組鼠纖維原生長因子-9( rmFGF-9) 2μg/10μg/1mg Prospec

 

 

Background :
 Fibroblast growth factor-9 (FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells, it is an autocrine/paracrine growth factor considered to be important for the growth and survival of motorneurons and prostate

 
Description :
 Recombinant Murine FGF-9 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 205 amino acids and having a molecular mass of 23308 Dalton.
Recombinant FGF9 is purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 20mM Tris pH=8 and 75mM NaCl.
Solubility:
 It is recommended to reconstitute the lyophilized FGF9 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized FGF-9 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution FGF9 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
 Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
 he sequence of the first five N-terminal amino acids was determined and was found to be
Pro-Leu-Gly-Glu-Val.

 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
 ProSpec’s rmFGF-9 is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2 x 106 Units/mg.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of Recombinant Human Fibroblast Growth Factor-basic.

 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a standard solution of FGF9 as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

佰易聚生物商城提供美國光譜醫學透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優質的分子生物學試劑和試劑盒、培養基本和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購!
 

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