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重組人GDP-5 (GDF-5)
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  • 廠商性質:經銷商
  • 更新時間:2025-05-02
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CYT-442重組人GDP-5 (GDF-5) 2μg/10μg/1mg Prospec

 

 

Background :
 
 
Description :
 Recombinant Human GDF-5 produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 117 amino acids and having a total molecular mass of 26.8 Dalton.
To enable bacterial expression of rhGDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added the Lys.
GDF5 is purified by proprietary chromatographic techniques.
 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.
 
Formulation:
 The protein was lyophilized without any additives.
 
Solubility:
 It is recommended to reconstitute the lyophilized Recombinant GDF-5 in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
 
Stability:
 Lyophilized GDF-5 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution rHuGDF-5 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
 
Purity:
 Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Arg-Gln-Gly.
 
Dimers and aggregates:
 ProSpec’s Human GDF-5 is fully biologically active when compared to standard.
Induction of alkaline phosphatase acrtivity in ATDC5 cells: EC50 = 40Nm.
 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of GDF-5.
 
Protein content:
 Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of GDF-5 as a Reference Standard.
 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


 佰易聚生物商城提供美國光譜醫學透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優質的分子生物學試劑和試劑盒、培養基本和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購!
 

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