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重組人白介素-19(rHuIL-19)
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  • 廠商性質:經銷商
  • 更新時間:2025-05-02
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CYT-363重組人白介素-19(rHuIL-19) 2μg/10μg/1mg Prospec

 

 

Background :
 Interleukin-19 (IL-19) is a novel cytokine that was initially identified during a sequence data base search aimed at finding potential Interleukin-10 homologs. Interleukin-19 shares 21% amino acid identity with IL-10. The exon/intron structure of IL-19 is similar to that of the human IL-10 gene, comprising five exons and four introns within the coding region of the IL-19 cDNA.
IL-19 shares a receptor complex with IL-20, indicating that the biological activities of these two cytokines overlap and that both may play an important role in regulating development and proper functioning of the skin. Unlike IL-10, which forms an intercalated dimer, the molecule of IL-19 is a monomer made of seven amphipathic helices, A-G, creating a unique helical bundle.
 
Description :
 Recombinant Human IL-19 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids and having a molecular mass of 17913 Dalton.

Recombinant Human Interleukin-19 is purified by proprietary chromatographic techniques.
 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.
 
Formulation:
 Lyophilized from a concentrated (1mg/ml) solution in water containing 10mM sodium citrate pH-5.
 
Solubility:
 It is recommended to reconstitute the lyophilized Interleukin-19 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
 
Stability:
 Lyophilized Interleukin-19 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Human IL-19 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
 
Purity:
 Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Cys.
 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
 
Biological Activity:
 ProSpec's recombinant Human Interleukin-19 is fully biologically active when compared to standard. The activity is determined by it's ability to activate STAT following receptor-ligand interaction.
 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of human IL-19.
 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of IL-19 as a Reference Standard.
 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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