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Name: 重組豬白介素-4（rPIL-4）
Price: 面議 CNY
Availability: InStock

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產品詳情

CYT-398

重組豬白介素-4（rPIL-4）

2μg/10μg/1mg

Prospec

Background :
	Interleukin-4 （IL4） has a compact, globular fold （similar to other cytokines）, stabilised by 3 disulphide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist. The helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. Interleukin （IL）-4, produced primarily by CD4（+） T cells is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.IL-4 has been known as a Th2 cytokine and can act on B cells, T cells, and monocytes.Interleukin （IL）-4 exhibits antitumor activity in rodent experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses.
	Description :
	Recombinant Porcine IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 110 amino acids and having a molecular mass of 12615 Dalton. Recombinant Porcine IL-4 is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	Lyophilized from a concentrated （1mg/ml） solution in water containing no additives.
	Solubility:
	It is recommended to reconstitute the lyophilized Porcine IL-4 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Recombinant Porcine IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution, IL-4 should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ly-Lys-Asp.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec’s Porcine IL-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of human TF-1 cells is < 10 ng/ml, corresponding to a Specific Activity of 1 x 10⁵ IU/mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of Porcine IL-4.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.218 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

佰易聚生物商城提供美國光譜醫學透析袋，wako生化試劑，amresco生化試劑，sigma生化試劑，優質的分子生物學試劑和試劑盒、培養基本和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄 www.ebioeasy.com或者,，洽談選購！

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上一篇：重組人白介素-4（rHuIL-4）
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